Terms and Definitions

Microscope: a machine for magnifying objects that space too small to be seen v the naked eye.

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basic microscope: solitary lens magnifier compound microscope: employs 2 or much more lenses

Parfocal: the objective lenses are placed on the microscope so that they have the right to be interchanged without having actually to appreciably differ the focus.

Resolving strength or resolution: the capacity to differentiate objects that are close together. The far better the fixing power that the microscope, the closer together two objects deserve to be and also still be viewed as separate.

Magnification: the procedure of enlarging the dimension of one object, together an optical image.

Total magnification: In a compound microscopic lense the full magnification is the product the the objective and ocular lenses (see figure below). The magnification the the ocular lenses on your scope is 10X.

objective lens X Ocular lens = Total magnification For example: low power: (10X)(10X) = 100X high dry: (40X)(10X) = 400X oil immersion: (100X)(10X) = 1000X

Immersion Oil: Clear, carefully detailed images are completed by difference the specimen with their medium. Transforming the refractive index of the specimens native their medium attains this contrast. The refractive index is a measure up of the relative velocity in ~ which irradiate passes through a material. As soon as light rays pass through the two materials (specimen and also medium) the have different refractive indices, the rays readjust direction from a straight path by bending (refracting) at the boundary between the specimen and the medium. Thus, this rises the image’s contrast between the specimen and also the medium.

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One way to adjust the refractive table of contents is by staining the specimen. Another is to usage immersion oil. While we want light come refract differently between the specimen and the medium, we do not desire to lose any kind of light rays, together this would decrease the resolution that the image. By put immersion oil in between the glass slide and the oil immersion lens (100X), the irradiate rays in ~ the highest possible magnification have the right to be retained. Immersion oil has the same refractive index as glass so the oil becomes component of the optics the the microscope. Without the oil the light rays room refracted as they enter the air between the slide and also the lens and the target lens would have to be enhanced in diameter in bespeak to capture them. Utilizing oil has actually the same result as increasing the target diameter thus improving the solving power that the lens.

Lab 1 / Ubiquity of microorganisms / Microscopy

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